human fibroblast cell line wi38  (JCRB Cell Bank)

Journal: BMC Complementary Medicine and Therapies

Article Title: Marine actinobacterium Streptomyces vinaceusdrappus mediated nano-selenium: biosynthesis and biomedical activities

doi: 10.1186/s12906-025-05073-9

Figure Lengend Snippet: Cell viability of Wi38, Caco-2, and PANC-1 cells treated with biogenic Se-NPs (31.2–1000 µg mL –1 ). Different letters ( a , b , and c ) on the bars at the same concentration indicate the results are significantly different ( n = 3, P ≤ 0.05)

Article Snippet: To assess the cytotoxic activity of Se-NPs using crystal violet viability assay, three different cell lines were investigated: the WI38 human fibroblast cell line (ATCC CCL-75), the Caco-2 human colon adenocarcinoma cell line (ATCC HTB-37), and the PANC-1 human pancreatic cancer cell line (ATCC CRL-1469).

Techniques: Concentration Assay

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Seeding approaches for tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds: (A) Wi38 monoculture, (B) Calu-3 monoculture, and (C) coculture.

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques:

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Seeding of the OL of tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds using Wi38 cells. (A) Cell metabolic activity of Wi38 cells on the OL and IL on days 1, 5, and 7. (B) DNA quantification on the OL and IL at day 7. (C) Coverage of the IL by Wi38 cells at day 7. Representative images of Wi38 cells growing on the OL using three seeding densities: (D) 1.5 × 10 5 cells/cm 2 , (E) 3 × 10 5 cells/cm 2 and (F) 6 × 10 5 cells/cm 2 . Cells were treated with DAPI (blue) and phalloidin (red) to stain the nuclei and actin filaments, respectively. Images were captured via confocal microscopy (Axio Examiner.Z1 confocal microscope). Results displayed as mean ± SEM ( n = 3, * p < 0.05, ** p < 0.01).

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques: Activity Assay, Staining, Confocal Microscopy, Microscopy

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Coculture on tubular bilayered PCL-Collagen and Hyaluronic acid (t-PCL-CHyA-B) scaffolds seeded on the outer layer (OL) with Wi38 cells and the inner layer (IL) with Calu-3 cells for 4h under rotation using 3D printed accessories. (A) Cell metabolic activity at days 2, 7, and 10 of culture. (B) Coverage of the IL at day 10 of culture. Representative images of the IL of t-PCL-CHyA-B scaffolds seeded with (C) Wi38 monoculture, (D) Calu-3 monoculture and (E) coculture using 3D printed accessories. Images were obtained using a Axio Examiner. Z1 confocal microscope. Cells were stained with DAPI (blue) to label nuclei and phalloidin (red) to stain actin filaments. Results displayed as mean ± SEM ( n = 3, ** p < 0.01, **** p < 0.0001).

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques: Activity Assay, Microscopy, Staining

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Coculture on tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds seeding the OL with Wi38 cells and the IL with Calu-3 cells for 2 h under rotation. (A) Cell metabolic activity at days 2, 7, and 10 of culture. (B) Coverage of the IL at day 10 of culture. Representative images of the IL of t-PCL-CHyA-B scaffolds: (D) Wi38 monoculture, (E) Calu-3 monoculture, and (F) coculture. Images were obtained using an Axio Examiner Z1 confocal microscope. Cells were stained with DAPI (blue) to label nuclei and phalloidin (red) to stain actin filaments. Results displayed as mean ± SEM ( n = 3).

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques: Activity Assay, Microscopy, Staining

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Cancer-associated fibroblasts promote pro-tumor functions of neutrophils in pancreatic cancer via IL-8: potential suppression by pirfenidone

doi: 10.1007/s00262-025-03946-z

Figure Lengend Snippet: iCAFs increase the migratory ability of neutrophils, activating them, and prolonging their life span. A WI38 cells were cultured in cancer CM for 4 days to produce CAFs. B α SMA and IL-8 are representative markers of myCAF and iCAF, respectively. Left: α SMA expression within CAFs induced by MIA PaCa-2-CM was lower than TGF-β-treated CAFs and non-treated control WI38 cells. Right: IL-8 expression within MIA PaCa-2-CM-induced CAFs was higher than in non-treated WI38 cells. C Migration assay of neutrophils. Image taken at 100 × magnification and the number of migrated cells per field was counted. Neutrophil migration was significantly increased in the CAF-CM group compared to the FBS-treated positive control. Mean ± SD is shown. Each dot represents the number of cells per field (× 100). One-way ANOVA with Tukey’s test was used. ***, P = 0.0004. D Compared to non-stimulated neutrophils, those treated with CAF-CM for 24 h showed increased intracellular IL-8. E. Comparing the area of living neutrophils in 9 random fields of view after 24 h, CAF-CM-stimulated neutrophils had a significantly larger area. Student’s t-test was used. ****, P < 0.0001

Article Snippet: The human fibroblast cell line WI38 was obtained from the Japanese Collection of Research Bioresources.

Techniques: Cell Culture, Expressing, Control, Migration, Positive Control